Gelatinase, 72 kDa
GELATINASE A
CAS: 193807-58-8
Molecular Formula: C21H19NO4S
Gelatinase, 72 kDa - Names and Identifiers
Gelatinase, 72 kDa - Physico-chemical Properties
| Molecular Formula | C21H19NO4S |
| Molar Mass | 381.44 |
| Appearance | powder |
| Color | light brown |
| Storage Condition | 2-8°C |
Gelatinase, 72 kDa - Risk and Safety
| Risk Codes | R36/37/38 - Irritating to eyes, respiratory system and skin. R42 - May cause sensitization by inhalation |
| Safety Description | S23 - Do not breathe vapour. S26 - In case of contact with eyes, rinse immediately with plenty of water and seek medical advice. S36 - Wear suitable protective clothing. S24/25 - Avoid contact with skin and eyes. |
| WGK Germany | 2 |
| FLUKA BRAND F CODES | 10-21 |
Gelatinase, 72 kDa - References
| Definition | the chemical name of Collagenase is collagen hydrolase (Collagenase), it can specifically hydrolyze the three-dimensional helical structure of natural collagen under physiological PH and temperature conditions without damaging other proteins and tissues. The chemical nature of collagenase is a protein, and therefore, it is very sensitive to temperature, PH, and various factors that cause protein denaturation, it is easily affected by external conditions and changes its conformation and properties. collagenase is an enzyme that specifically degrades the helical region of collagen or gelatin without acting on other protein substrates. |
| classification of collagenase | according to its existence in different ways can be divided into human endogenous collagenase and medicinal collagenase two kinds. Human endogenous collagenase refers to the collagenase in the human body itself, such as gingival, membrane and other epithelial tissue and joint synovial, it plays an integral role in the breakdown of collagen in the body. medicinal collagenase refers to a white or off-white sterile lyophilized powder biological preparation obtained by extracting, purifying and refining from the fermentation broth of Clostridium histolytica by high-tech means of Biopharmaceutics. The medicinal collagenase is divided into type I, II, IV and V, and the type of collagenase should be selected according to the type of tissue to be separated and digested. |
| configuration and use | collagenase (collagenase) was prepared from Clostridium histolytica, it mainly hydrolyzes the collagen component in connective tissue. When the tissue to be digested is hard and contains more connective tissue or collagen components, the effect of dissociating cells with trypsin is poor, and collagenase dissociation method can be used at this time. Collagenase only had a digestive effect on the intercellular substance and had little effect on the epithelial cells. Therefore, it is suitable for digesting and separating fibrous tissue, epithelial and cancerous tissue, and can separate epithelial cells from collagen components without being damaged. The usual dose is a final concentration of 200U/ml (about 1 mg/ml) or 0.03-0.3. Collagenase is divided into I, II, III, IV, V type and liver cell specific collagenase, according to the type of tissue to be separated and digested to select the type of collagenase. 1. Collagenase I is used for the separation of epithelial, lung, adipose and adrenal tissue cells. It is used to digest the connective moiety in the tissue to make it into a single cell for the isolation of mammalian cells. 2. Collagenase IV contains at least 7 kinds of protease components, and the molecular weight varies from 68 to 130KD. It can digest a variety of tissues. 3. Collagenase V contains at least 7 protease components with molecular weights ranging from 68-130KD. It can be used for the isolation of pancreatic islet tissue, separating connective tissue into single cells. 4. Collagenase II is applicable to liver, bone, thyroid, heart and salivary gland tissues. |
Last Update：2024-04-09 21:32:14
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